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Human Embryonic Stem Cell Lines Generated Without Destruction

By Young Chung, Irina Klimanskaya, Sandy Becker, Tong Li, Marc Maserati, Shi-Jiang Lu, Tamara Zdravkovic, Dusko Ilic, Olga Genbacev, Susan Fisher, Ana Krtolica, and Robert Lanza

To date, the derivation of all human embryonic stem cell (hESC) lines has involved destruction of embryos. We previously demonstrated that hESCs can be generated from single blastomeres (Klimanskaya et al., 2006). In that ‘‘proof-of-principle’’ study, multiple cells were removed from each embryo and none of the embryos were allowed to continue development. Here we report the derivation of five hESC lines without embryo destruction, including one without hESC coculture. Single blastomeres were removed from the embryos by using a technique similar to preimplantation genetic diagno- sis (PGD). The biopsied embryos were grown to the blastocyst stage and frozen. The blastomeres were cultured by using a modified approach aimed at recreating the ICM niche, which substantially improved the efficiency of thehESC derivation to rates comparable to whole embryo derivations. All five lines maintained nor- mal karyotype and markers of pluripo- tency for up to more than 50 passages and differentiated into all three germ layers.

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Robert Lanza takes the Ice Bucket Challenge

Robert Lanza Article Thumbnail Graphic

"Before I did the #icebucketchallenge, I challenged the leader of Advanced Cell Technology (ACT), Dr. Bob Lanza, to do the Ice Bucket Challenge. He did it and leading up to it he provided a quite articulate message for context (see video). Bob is one very cool guy even without ice water."
Paul Knoepfler

Entire Company takes the Ice Bucket Challenge. Click Here to See Video.